Before moving forward with any of the steps below please Start your project. This will provide us with the necessary information to start your project. We will then get back to you with specific information regarding sample requirement, permits etc…
Sample Collection and Preservation:
Flash freezing samples and storing at -80C is the optimal preservation method for long-read technologies. For nucleated blood, we recommend an ethanol collection protocol.
Please note, most lysis and stabilizing buffers (such as RNAlater) are NOT compatible with our long-read technologies.
This is work in progress and will certainly evolve. For genomics, these are our preferred sample types (in order of priority):
Mammals: (1) cells, (2) spleen, (3) muscle, (4) blood
Birds: (1) cells, (2) blood, (3) muscle
Reptiles: (1) cells, (2) blood, (3) muscle
Amphibians: (1) cells, (2) blood, (3) muscle, (4) whole body (skin and digestive system dissected out)
Bony fishes: (1) cells, (2) heart, (3) blood, (4) gills, (5) muscle
Cartilaginous fishes: (1) cells, (2) blood, (3) gills, (4) muscle
For transcriptomics: (1) brain, (2) gonads, (3) lungs, (4) liver, (5) muscle, (6) blood, (7) cells.
See our preprint for more information: "Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing". https://doi.org/10.1101/2021.07.13.451380
If any of the requirements above are not realistic for your organism, let us know and we can look into alternative strategies.
Please read through the full guide to ensure all protocol is met for packaging your samples
Packaging Non-Nucleated Blood
Packaging Nucleated Blood
Additional notes: Plastic boxes are prone to shattering when frozen and are not recommended for use. Include absorbent material around blood in packaging if available. If you are shipping mammal blood on the same day as collection, it can be shipped fresh on ice packs instead of dry ice when shipping from within the US.
The outside of your shipping box must prominently display a UN 1845 dry ice sticker and a sticker or marking indicating the package must be kept upright
Check with us about potential permit requirements (especially for international shipment). We have a USDA permit to import bird samples and CITES permit for all vertebrates.
Please send us information about your species of interested (scientific name, origin etc...) as soon as possible. so that we can apply to additional permits if needed.